Cell Culture Media Preparation Calculator

Calculate volumes of base media, serum, and supplements needed to prepare cell culture media for a given total volume with correct percentages.

Total Volume to Prepare (mL)

Serum Percentage (%)

Antibiotic Percentage (%)

L-Glutamine Percentage (%)

Other Supplements (%)

Formula

Base Media = Total Volume x (100 - Sum of Supplement %) / 100; Supplement Volume = Total Volume x Supplement % / 100

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