DNA Concentration Calculator
From A260 Absorbance
Calculate DNA concentration from A260 reading
Total DNA Yield
Calculate total DNA yield from concentration and volume
Formula
Concentration (µg/mL) = A260 × Factor × Dilution Factor. dsDNA factor = 50, ssDNA = 33, RNA = 40. Total yield = concentration × volume. 1 A260 unit of dsDNA ≈ 50 µg/mL.
Frequently Asked Questions
How do you calculate DNA concentration from A260?
DNA concentration = A260 × conversion factor × dilution factor. For dsDNA the factor is 50 µg/mL per A260 unit, for ssDNA it is 33, and for RNA it is 40.
What is a good 260/280 ratio?
A 260/280 ratio of ~1.8 is generally accepted as pure DNA, while ~2.0 indicates pure RNA. Ratios significantly lower suggest protein, phenol, or other contaminant presence.
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