Michaelis-Menten Kinetics Calculator
Calculate Reaction Velocity
Find V from substrate concentration, Vmax, and Km
Estimate Km from Two Data Points
Estimate Km and Vmax from two velocity-substrate pairs
Formula
V = Vmax × [S] / (Km + [S]). Lineweaver-Burk: 1/V = (Km/Vmax)(1/[S]) + 1/Vmax. Catalytic efficiency = kcat/Km.
Frequently Asked Questions
What is the Michaelis-Menten equation?
The Michaelis-Menten equation describes enzyme kinetics: V = Vmax × [S] / (Km + [S]). Vmax is the maximum velocity and Km is the Michaelis constant — the substrate concentration at which V = Vmax/2.
What does Km represent?
Km (Michaelis constant) is the substrate concentration at which the reaction velocity is half of Vmax. A low Km indicates high affinity for the substrate, while a high Km indicates low affinity.
What is catalytic efficiency?
Catalytic efficiency is kcat/Km and measures how efficiently an enzyme converts substrate to product. The theoretical upper limit is the diffusion limit (~10⁸–10⁹ M⁻¹s⁻¹).
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