Spectrophotometer Calculator
Spectrophotometer
Calculate the concentration of a solution using Beer-Lambert law from absorbance and path length data
AU
L/mol cm
cm
Formula
Concentration = Absorbance / (Molar Absorptivity x Path Length)
Frequently Asked Questions
What is the Beer-Lambert law?
The Beer-Lambert law states that absorbance (A) is directly proportional to the concentration (c) of the absorbing species, the path length (l), and the molar absorptivity (epsilon): A = epsilon x l x c.
What are the limitations of the Beer-Lambert law?
The Beer-Lambert law is most accurate at low concentrations. At high concentrations, deviations occur due to molecular interactions, scattering, and detector saturation, typically above an absorbance of 2.