Western Blot Antibody Dilution Calculator

Western Blot Antibody Dilution

Calculate antibody dilution volumes for western blot experiments based on stock concentration, desired working dilution, and total volume of blocking solution.

Desired Dilution (e.g. 1000 for 1:1000)

Total Solution Volume (mL)

Stock Antibody Conc. (mg/mL)

Number of Membrane Strips

Formula

Antibody Volume (mL) = Total Volume / Dilution Factor; Working Conc. (ug/mL) = Stock Conc. (mg/mL) x 1000 / Dilution; Blocking Solution = Total Volume - Antibody Volume

Frequently Asked Questions

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