Bradford Assay Protein Concentration Calculator

Bradford Assay Protein Concentration

Calculate protein concentration from Bradford assay absorbance readings using a standard curve with BSA standards and Beer-Lambert law.

Sample Absorbance (595 nm)

Standard Curve Slope (Abs/ug/mL)

Standard Curve Y-Intercept

Sample Dilution Factor

Total Sample Volume (mL)

Formula

Concentration (diluted) = (Absorbance - Intercept) / Slope; Original Concentration = Diluted Conc. x Dilution Factor; Total Protein = Concentration x Volume

Frequently Asked Questions

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