FullCalculator

Serial Dilution Calculator

Serial Dilution Series

Calculate concentrations at each step of a serial dilution

Back-Calculate Original Concentration

Find original concentration from a diluted sample count

Formula

Final concentration = C₀ / (DF)ⁿ, where C₀ is initial concentration, DF is dilution factor per step, and n is the number of steps. Total dilution = DFⁿ.

Frequently Asked Questions

What is a serial dilution?
A serial dilution is a stepwise dilution of a substance in solution, where the diluted material from one step is used to make the next dilution. For example, six 1:10 serial dilutions produce a total dilution of 1:1,000,000.
How do you calculate the final concentration in a serial dilution?
Final concentration = Initial concentration / (dilution factor)^n, where n is the number of steps. For a 1:10 dilution repeated 4 times: final = initial / 10⁴ = initial / 10,000.

You may also need