Cell Dilution Calculator

Find Volume of Stock Needed

How much stock solution to add to reach a target concentration

Stock Concentration (C₁, cells/mL)

Target Concentration (C₂, cells/mL)

Final Volume (V₂, mL)

Find Final Concentration

Calculate resulting concentration after dilution

Stock Concentration (C₁, cells/mL)

Volume of Stock (V₁, mL)

Final Volume (V₂, mL)

Formula

C₁ × V₁ = C₂ × V₂. V₁ = (C₂ × V₂) / C₁. Dilution factor = C₁ / C₂.

Frequently Asked Questions

What is the dilution equation?

The dilution equation is C₁V₁ = C₂V₂, where C₁ is the initial concentration, V₁ is the volume of stock, C₂ is the final concentration, and V₂ is the final total volume.

How do you calculate dilution factor?

Dilution factor = initial concentration / final concentration, or equivalently, final volume / volume of stock. A 1:10 dilution means the final concentration is 1/10 of the stock.

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