Hemocytometer Cell Count Calculator
Standard Hemocytometer Count
Calculate cell concentration from a standard hemocytometer counting
With Viability (Trypan Blue)
Calculate cell concentration and viability using trypan blue exclusion
Formula
Cells/mL = (Average cells per square) × (Dilution factor) × 10,000. Viability (%) = (Live cells / Total cells) × 100.
Frequently Asked Questions
What is a hemocytometer?
A hemocytometer (Neubauer chamber) is a specialized glass microscope slide with an etched grid used to count cells. Each large square has a defined volume (typically 0.1 µL), allowing calculation of cell concentration.
How do you calculate cell concentration with a hemocytometer?
Cell concentration (cells/mL) = (average cells per square) × (dilution factor) × 10,000. The factor of 10,000 converts from the 0.1 µL chamber volume to 1 mL.
What is trypan blue exclusion?
Trypan blue is a dye that penetrates dead cells (with compromised membranes) but is excluded by living cells. Counting stained vs. unstained cells gives cell viability percentage.
You may also need
A
Cell Dilution Calculator
Free cell dilution calculator. Calculate the volume of cell stock and diluent needed to achieve a target cell concentration using C1V1 = C2V2.
ScienceA
Serial Dilution Calculator
Free serial dilution calculator. Calculate the final concentration after multiple sequential dilution steps.
ScienceA
Generation Time Calculator
Free bacterial generation time calculator. Calculate doubling time, growth rate, and final population from initial conditions.
Science