Cell Viability Calculator

Cell Viability

Calculate cell viability percentage and viable cell concentration from trypan blue exclusion assay hemocytometer counts in cell culture experiments.

Live Cells Counted

Dead Cells Counted (Stained)

Large Squares Counted

Dilution Factor

Formula

Viability (%) = (Live Cells / Total Cells) x 100; Cells/mL = (Avg Cells/Square) x 10,000 x Dilution Factor; Viable Cells/mL = Cells/mL x (Viability/100)

Frequently Asked Questions

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