OD600 Cell Density Calculator

Convert optical density at 600nm (OD600) readings to estimated cell density in CFU/mL or cells/mL for common bacterial and yeast cultures.

OD600 Reading

Organism Type

Dilution Factor

Target OD600 for Subculture

Formula

Cell Density = OD600 x Dilution Factor x Conversion Factor; Dilution for Target = Actual OD / Target OD; E. coli: ~8 x 10^8 cells/mL per OD unit

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