Primer Melting Temperature (Tm) Calculator

Calculate DNA primer melting temperature using basic, salt-adjusted, and nearest-neighbor methods for PCR primer design and optimization.

Primer Length (bp)

Number of G+C Bases

Na+ Concentration (mM)

Primer Concentration (nM)

Formula

Basic Tm = 2(A+T) + 4(G+C); Salt-Adjusted Tm = 100.5 + 41(GC/N) - 820/N + 16.6 x log10([Na+]); Annealing Temp ~ Tm - 5 C

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Calculate the theoretical DNA amplification yield from PCR based on number of cycles, initial template copies, and amplification efficiency.

Free PCR primer melting temperature calculator. Estimate Tm using the basic rule, salt-adjusted method, and nearest-neighbor approximation.

Free DNA concentration calculator. Convert between absorbance (A260), DNA concentration, and total yield for spectrophotometric measurements.