PCR Cycle Number Calculator

Calculate the theoretical DNA amplification yield from PCR based on number of cycles, initial template copies, and amplification efficiency.

Initial Template Copies

Number of PCR Cycles

Amplification Efficiency (%)

Formula

Final Copies = N0 x (1 + E)^n; where N0 = initial copies, E = efficiency (decimal); n = number of cycles; Fold Amplification = (1 + E)^n

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