qPCR Fold Change Calculator (Delta-Delta Ct)

qPCR Fold Change (Delta-Delta Ct)

Calculate relative gene expression fold change from qPCR data using the delta-delta Ct method comparing target and reference genes across treatment and control samples.

Ct Target Gene (Treatment)

Ct Reference Gene (Treatment)

Ct Target Gene (Control)

Ct Reference Gene (Control)

Formula

Delta Ct = Ct(target) - Ct(reference); Delta-Delta Ct = Delta Ct(treatment) - Delta Ct(control); Fold Change = 2^(-Delta-Delta Ct)

Frequently Asked Questions

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