Transformation Efficiency Calculator

Calculate bacterial transformation efficiency as colony forming units per microgram of plasmid DNA from transformation experiment plate counts.

Colonies on Plate

DNA Used (ng)

Total Recovery Volume (uL)

Volume Plated (uL)

Formula

Total Colonies = Counted Colonies x (Total Volume / Volume Plated); Efficiency (CFU/ug) = Total Colonies / DNA (ug)

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