DNA Ligation Ratio Calculator

Calculate the optimal insert-to-vector molar ratio and DNA masses for molecular cloning ligation reactions based on fragment sizes.

Vector Size (bp)

Insert Size (bp)

Vector Mass (ng)

Insert:Vector Molar Ratio

Formula

Insert mass (ng) = Ratio x Vector mass (ng) x Insert size (bp) / Vector size (bp); pmol = mass (ng) x 1000 / (size (bp) x 660)

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