DNA/RNA Yield and Purity Calculator

DNA/RNA Yield and Purity

Calculate nucleic acid concentration, total yield, and purity ratios from UV spectrophotometer A260/A280 readings for DNA and RNA samples.

Absorbance at 260 nm

Absorbance at 280 nm

Absorbance at 230 nm

Nucleic Acid Type

Dilution Factor

Total Sample Volume (uL)

Formula

Concentration = A260 x Conversion Factor x Dilution; Total Yield = Concentration x Volume / 1000; Conversion: dsDNA = 50, RNA = 40, ssDNA = 33 (ug/mL per OD260)

Frequently Asked Questions

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