ELISA Standard Curve Calculator

Calculate analyte concentration from ELISA optical density readings using a linear standard curve with known concentration standards.

Sample OD Reading

Standard Curve Slope

Y-Intercept

Sample Dilution Factor

Concentration Unit

Formula

Concentration (diluted) = (Sample OD - Intercept) / Slope; Original Concentration = Diluted Conc. x Dilution Factor

undefined

Calculate protein concentration from Bradford assay absorbance readings using a standard curve with BSA standards and Beer-Lambert law.

Calculate the concentration of a solution using Beer-Lambert law from absorbance and path length data.

Free serial dilution calculator. Calculate the final concentration after multiple sequential dilution steps.