Gel Electrophoresis Band Size Estimator

Estimate DNA or protein band molecular weight from gel electrophoresis migration distance using a standard curve from known molecular weight markers.

Marker Band 1 Size (bp or kDa)

Marker 1 Migration (mm)

Marker Band 2 Size (bp or kDa)

Marker 2 Migration (mm)

Sample Band Migration (mm)

Formula

log10(Size) = Slope x Distance + Intercept; Slope = (log(Size2) - log(Size1)) / (Dist2 - Dist1); Estimated Size = 10^(log10(Size))

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