Plasmid Copy Number Calculator

Plasmid Copy Number

Estimate plasmid copy number per cell from qPCR or gel densitometry data by comparing plasmid DNA signal to chromosomal DNA reference signal.

Plasmid Gene Ct Value

Chromosomal Gene Ct Value

PCR Efficiency (%)

Chromosomal Gene Copies Per Genome

Formula

Copy Number = E^(Ct_chrom - Ct_plasmid) x Chromosomal Copies; where E = 1 + (Efficiency/100); Delta Ct = Ct(chromosomal) - Ct(plasmid)

Frequently Asked Questions

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