Transfection Efficiency Calculator

Transfection Efficiency

Calculate mammalian cell transfection efficiency and optimize DNA-to-reagent ratios for lipofection, electroporation, or calcium phosphate transfection methods.

Total Cells Counted

Positive (Transfected) Cells

DNA Amount (ug)

Transfection Reagent (uL)

Well Area (cm2)

Formula

Transfection Efficiency (%) = (Positive Cells / Total Cells) x 100; Reagent:DNA Ratio = Reagent Volume (uL) / DNA Amount (ug); DNA/cm2 = DNA Amount / Well Area

Frequently Asked Questions

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